Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Expression levels of 17 genes based on GEPIA2 (Gene Expression Profiling Interactive Analysis web server). (A) ANGPTL6 . (B) CFP . (C) CLEC1B . (D) CLEC4G . (E) CLEC4M . (F) COLEC10 . (G) CRHBP . (H) CXCL12 . (I) DNASE1L3 . (J) FCN2 . (K) FCN3 . (L) GSTZ1 . (M) LCAT . (N) NAT2 . (O) OIT3 . (P) RSPO3 . (Q) VIPR1 . * p < 0.05. The y -axis represents the relative log2 expression value (TPM + 1).

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Survival analysis of 17 genes. (A) ANGPTL6 . (B) CFP . (C) CLEC1B . (D) CLEC4G . (E) CLEC4M . (F) COLEC10 . (G) CRHBP . (H) CXCL12 . (I) DNASE1L3 . (J) FCN2 . (K) FCN3 . (L) GSTZ1 . (M) LCAT . (N) NAT2 . (O) OIT3 . (P) RSPO3 . (Q) VIPR1 . (R) Constructed protein–protein interaction (PPI) network of the important differentially expressed genes (DEGs) using STRING. (S) Use of the Cytoscape plug-in MCODE to select the most important module from the PPI network.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Construct

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Pan-tissue expression of FCN2 . (A) Pan-cancer expression of FCN2 in the Oncomine database. (B) Expression of FCN2 in normal tissues in the BioGPS database. (C) Pan-cancer expression of FCN2 in the UCSC (University of California, Santa Cruz) database. (D) Log2 transformation of the pan-cancer expression value of FCN2 in the UCSC database. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not statistically significant.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Expressing, Transformation Assay

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Transcription levels of FCN2 in liver cancer and normal tissues. (A–C) Expression differences of the FCN2 gene between liver cancer tissues and normal tissues in the Oncomine database. (D) FCN2 mRNA expression in liver cancer tissue and normal tissue based on the GEPIA2 database (TCGA tumors vs . TCGA normal). (E, F) FCN2 protein expression in hepatocellular carcinoma in the UALCAN database. (G) Part of the results of the immunohistochemistry experiments. (H) Average optical density of liver cancer tissues and adjacent tissues from 30 liver cancer patients. TCGA , The Cancer Genome Atlas. * p < 0.05, *** p < 0.001.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Expressing, Immunohistochemistry

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Different expression levels of FCN2 in liver hepatocellular carcinoma (LIHC) based on The Cancer Genome Atlas (TCGA) analyzed using R. (A) Pathologic stages. (B) Histological grade. (C) Fibrosis Ishak scale scores. (D) Race. (E) Normal tissue and liver cancer. (F) Adjacent hepatic tissue inflammation. (G) Age (H) . BMI. (I) Alpha-fetoprotein (AFP). (J) Vascular invasion. (K) Gender. * p < 0.05, ** p < 0.01, *** p < 0.001. ns: not statistically significant.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Prognostic value of FCN2 in liver cancer. (A–D) Overall survival (OS) (A) , relapse-free survival (RFS) (B) , disease-specific survival (DSS) (C) , and patient-free survival/progression-free survival (PFS) (D) . (E) Nomogram predicting the 1-, 3-, and 5-year OS probability. (F) C-index of the prognostic model nomogram for predicting the 1-, 3-, and 5-year OS probability in hepatocellular carcinoma (HCC) patients. (G) Prediction of the 1-, 3-, and 5-year OS probability using nomogram calibration plots.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques:

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Protein–protein interaction network analysis and enrichment analysis. (A, B) Gene network diagram of interaction with FCN2 created using the STRING database. (C–G) Diagrams of the analyses of the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) pathways.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques:

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Correlation of FCN2 expression with immune characteristics. (A) Differential distribution of the immune cells in patients with high and low FCN2 expressions. (B–G) Correlation between the expression level of FCN2 and immune infiltration in hepatocellular carcinoma: (B) Neutrophils, (C) Eosinophils, (D) NK cells, (E) Tcm, (F) DC, (G) Th2 cells. * p < 0.05, ** p < 0.01, *** p < 0.001. ns, no significance.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Correlation analysis between FCN2 and the biomarkers of immune cells in hepatocellular carcinoma (HCC) determined using the GEPIA database (Spearman’s correlation coefficient).

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques:

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Immunomodulators, chemokines, and receptors associated with FCN2 . (A) Distribution of the FCN2 immunological scores in tumor and normal tissues.The ordinate reflects the distribution of the immunological scores in distinct groups, whereas the abscissa indicates the immune cell types. The Wilcoxon test was used to compare statistical differences between the two groups, and the Kruskal–Wallis test was used to determine the significance of the differences between three groups. ( a ) Heatmap of the immune cell scores. Different hues represent the varied expression distributions in different samples. Asterisks indicate significance levels at * p < 0.05, ** p < 0.01, and *** p < 0.001. ( b ) Percentages of tumor-infiltrating immune cells in each sample. Different colors depict the different types of immunological cells. The abscissa denotes the sample, whereas the ordinate denotes the percentage of immune cells in a single sample. (H) Immunomodulators, chemokines, and receptors associated with FCN2 in liver hepatocellular carcinoma (LIHC). (B) CCL14. (C) CCL16. (D) CCL23. (E) TNFSF4. (F) CD276. (G) TNFRSF4. (H) KDR. **** p < 0.0001.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Immunohistochemical comparison of FCN2 and the molecules with strong correlations [based on the Human Protein Atlas (HPA)].

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Immunohistochemical staining

Journal: PLoS ONE

Article Title: A Novel Peptide Enhances Therapeutic Efficacy of Liposomal Anti-Cancer Drugs in Mice Models of Human Lung Cancer

doi: 10.1371/journal.pone.0004171

Figure Lengend Snippet: ( A ) A phage-displayed random peptide library was used to select phages that bind to the NSCLC cell line CL1-5. ( B ) Visualization of PC5-2 binding to CL1-5 and PC13 lung cancer cells (arrowheads) with immunohistochemical staining. The control phage did not bind to CL1-5 cells. Scale bar: 10 µm. ( C ) The FITC-labeled peptide SP5-2 bound to five NSCLC cell lines but not to NPC-TW01 cells as detected by immunofluorescent staining. Scale bar: 10 µm. ( D ) Representative photomicrographs of tumor sections from surgical specimens of human lung cancer were detected using both PC5-2 (a, arrowhead) and biotinylated SP5-2 (c, arrowhead), respectively. In comparison, the control phage or biotinylated control peptide could not bind to these surgical specimens (b and d). PC5-2 was competitively inhibited by the synthetic peptide SP5-2 (e). Mutated peptide, MP5-2, lost this competition ability (f). Scale bar: 25 µm.

Article Snippet: The biotinylated lectin was visualized with streptavidin-conjugated rhodamine (Pierce, IL, USA).

Techniques: Binding Assay, Immunohistochemical staining, Staining, Control, Labeling, Comparison

Journal: PLoS ONE

Article Title: A Novel Peptide Enhances Therapeutic Efficacy of Liposomal Anti-Cancer Drugs in Mice Models of Human Lung Cancer

doi: 10.1371/journal.pone.0004171

Figure Lengend Snippet: ( A ) Representative two-color images showing the distribution of doxorubicin (red) in relation to nuclei (blue) in tissue sections. Accumulation of doxorubicin in tumor tissues was shown at 1, 4 and 24 hours post-injection. Bar, 50 µm. ( B ) Histopathology and fluorescent staining of tumor tissues in each treatment group examined after staining with H&E, TUNEL (green), lectin (red), and DAPI (blue). Bar, 50 µm. Enhancement of drug accumulation in tumor tissues correlated with the increased therapeutic efficacy.

Article Snippet: The biotinylated lectin was visualized with streptavidin-conjugated rhodamine (Pierce, IL, USA).

Techniques: Injection, Histopathology, Staining, TUNEL Assay, Drug discovery

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Macrophage Infiltration Reduces Neurodegeneration and Improves Stroke Recovery after Delayed Recanalization in Rats

doi: 10.1155/2022/6422202

Figure Lengend Snippet: Angiogenesis after delayed recanalization. (a) Representative image of microvessels stained with lectin in sham, rMCAO, and pMCAO groups. (b) Representative immunohistochemical staining for lectin, PDGFR β , and CD34. Angiogenesis is depicted by cells expressing colocalization of PDGFR β and CD34 markers around the endothelial cells. (c) Representative immunohistochemical staining for fibronectin, PDGFR β , and CD34; BBB leakage is depicted by fibronectin expression around microvessels. (d) Quantification of microvessel area in each group. (e–g) Quantification of CD34-positive cells, pericyte coverage, and BBB leakage in each group. Error bars represent the mean ± SD. ∗ P < 0.05 and ∗∗ P < 0.01 vs. sham group; # P < 0.05 and ## P < 0.01 vs. rMCAO group ( n = 5).

Article Snippet: Lectin , VectorLab (B-1175-1) , 1 : 500 (IHC), 1 : 200 (IF).

Techniques: Staining, Immunohistochemical staining, Expressing

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Macrophage Infiltration Reduces Neurodegeneration and Improves Stroke Recovery after Delayed Recanalization in Rats

doi: 10.1155/2022/6422202

Figure Lengend Snippet: Macrophage depletion reduces angiogenesis after delayed recanalization. (a) Representative images of macrophages via F4/80 immunohistochemical staining in the spleen. (b) Quantification of macrophage in each group. (c) Western blotting analysis of macrophage marker F4/80 expression. (d) Quantification of macrophage marker F4/80 expression in spleen. (e) Western blotting analyses of macrophage, neuroinflammation, and angiogenesis markers. (f–h) Quantification of related proteins in each group. (l) Representative images of microvessel density in the peri-infarction area visualized by immunohistochemical staining with lectin. Error bars represent the mean ± SD. ∗ P < 0.05 and ∗∗ P < 0.01 vs. sham group; # P < 0.05 and ## P < 0.01 vs. rMCAO group; & P < 0.05 and && P < 0.01 vs. rMCAO+PBS ( n = 5).

Article Snippet: Lectin , VectorLab (B-1175-1) , 1 : 500 (IHC), 1 : 200 (IF).

Techniques: Immunohistochemical staining, Staining, Western Blot, Marker, Expressing

Journal: bioRxiv

Article Title: Maternal group 2 innate lymphoid cells control fetal growth and protect from endotoxin-induced abortion in mice

doi: 10.1101/348755

Figure Lengend Snippet: (A) Stereological and immunohistochemical quantification of uterine artery thickness and lumen area ratios in the decidua of dams from ♀ILC2KO × ♂WT crosses and dams from ♀WT × ♂WT crosses at midgestation (gd9.5). Pooled data from 4 litters per cross, n = 12-20 total implantation sites per cross. (B) Smooth muscle actin (SMA) staining on decidual uterine arteries at midgestation (gd9.5) in dams from ♀WT × ♂WT and ♀ILC2KO × ♂WT crosses; 12.3X magnification, scale bar 100μm. (C) Relative mRNA expression of pro-inflammatory cytokines in the uterus of dams from ≀WT × ♂WT (n=4) and ♀ILC2KO × ♂WT (n=3) crosses at midgestation (gd9.5). (D) Relative mRNA expression of tissue glucose and amino acid transporters from dissected term placentas (gd18.5). of 4 ♀WT × ♂1LC2KO crosses (n = 12 placentas) and 5 ♀ILC2KO × ♂WT crosses (n = 13 placentas). (E) Representative photomicrographs of sections of placentas at term (gd18.5) stained for lectin, cytokeratin and eosin to highlight fetal vessels (FV), trophoblast (TB) and maternal blood spaces (MBS);. Data in A, C and D are displayed as mean ± SEM. Data in A were analysed by Mann-Whitney test, data in C and D were analysed by paired Student’s t -test.

Article Snippet: To assess Lz composition and component volumes, placental sections were doublelabelled with rabbit antibodies against cytokeratin (Thermo-Fisher Scientific 180059) and lectin (Vector Labs B-1205) to identify trophoblast and fetal capillaries respectively, and counterstained with haematoxylin and eosin.

Techniques: Immunohistochemical staining, Staining, Expressing, MANN-WHITNEY

Journal: bioRxiv

Article Title: Maternal group 2 innate lymphoid cells control fetal growth and protect from endotoxin-induced abortion in mice

doi: 10.1101/348755

Figure Lengend Snippet: Quantification of maternal blood spaces in sections of placentas at term (gd18.5) stained for lectin, cytokeratin and eosin, as shown (n=4 placentas/group). Data are displayed as means ± SEM and were analysed by Student’s t -test.

Article Snippet: To assess Lz composition and component volumes, placental sections were doublelabelled with rabbit antibodies against cytokeratin (Thermo-Fisher Scientific 180059) and lectin (Vector Labs B-1205) to identify trophoblast and fetal capillaries respectively, and counterstained with haematoxylin and eosin.

Techniques: Staining

Journal: Heliyon

Article Title: Luteolin reduces cardiac damage caused by hyperlipidemia in Sprague-Dawley rats

doi: 10.1016/j.heliyon.2023.e17613

Figure Lengend Snippet: MMP2 and MMP9 expression in the cardiac tissues of three groups of rats fed different diets. (A) Relative mRNA expression levels of MMP2 and MMP9 in the cardiac tissues of each group were measured after 12 weeks. Data are presented as the mean ± SEM; n = 6 in each group. * P < 0.05 vs HFD group. (B) Representative immunohistochemical staining for MMP2 and MMP9. Arrows indicate positively stained cells. (C) Bar graph showing MMP2 and MMP9 positive cells. Data are presented as the mean ± SEM; n = 3 in each group. * P < 0.05 vs HFD group.

Article Snippet: Next, the sections were blocked with 3% H 2 O 2 in methanol for 15 min to inactivate the endogenous peroxidases and incubated overnight at 4 °C with the primary antibodies: TGF-β (rabbit anti-TGF-β antibody, 1:300; Proteintech, Wuhan, China), collagen I (rabbit anti-collagen I antibody, 1:1000; Proteintech), collagen III (rabbit anti-collagen III antibody, 1:1000; Proteintech), MMP2 (rabbit anti- MMP2 antibody, 1:200; Proteintech), MMP9 (rabbit anti- MMP9 antibody, 1:300; Proteintech), LOX-1 (rabbit anti-LOX-1 antibody, 1:300; Abcam, England, CD36 (rabbit anti-CD36 antibody, 1:500; Proteintech).

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Heliyon

Article Title: Luteolin reduces cardiac damage caused by hyperlipidemia in Sprague-Dawley rats

doi: 10.1016/j.heliyon.2023.e17613

Figure Lengend Snippet: Primer oligonucleotide sequences.

Article Snippet: Next, the sections were blocked with 3% H 2 O 2 in methanol for 15 min to inactivate the endogenous peroxidases and incubated overnight at 4 °C with the primary antibodies: TGF-β (rabbit anti-TGF-β antibody, 1:300; Proteintech, Wuhan, China), collagen I (rabbit anti-collagen I antibody, 1:1000; Proteintech), collagen III (rabbit anti-collagen III antibody, 1:1000; Proteintech), MMP2 (rabbit anti- MMP2 antibody, 1:200; Proteintech), MMP9 (rabbit anti- MMP9 antibody, 1:300; Proteintech), LOX-1 (rabbit anti-LOX-1 antibody, 1:300; Abcam, England, CD36 (rabbit anti-CD36 antibody, 1:500; Proteintech).

Techniques: