immunohistochemical staining with lectin Search Results


biotinylated lectin  (Thermo Fisher)
99
Thermo Fisher biotinylated lectin
( A ) A phage-displayed random peptide library was used to select phages that bind to the NSCLC cell line CL1-5. ( B ) Visualization of PC5-2 binding to CL1-5 and PC13 lung cancer cells (arrowheads) with immunohistochemical staining. The control phage did not bind to CL1-5 cells. Scale bar: 10 µm. ( C ) The FITC-labeled peptide SP5-2 bound to five NSCLC cell lines but not to NPC-TW01 cells as detected by immunofluorescent staining. Scale bar: 10 µm. ( D ) Representative photomicrographs of tumor sections from surgical specimens of human lung cancer were detected using both PC5-2 (a, arrowhead) and <t>biotinylated</t> SP5-2 (c, arrowhead), respectively. In comparison, the control phage or biotinylated control peptide could not bind to these surgical specimens (b and d). PC5-2 was competitively inhibited by the synthetic peptide SP5-2 (e). Mutated peptide, MP5-2, lost this competition ability (f). Scale bar: 25 µm.
Biotinylated Lectin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ihc vector labs dl 1178 1 lectin dylight  (Vector Laboratories)
96
Vector Laboratories ihc vector labs dl 1178 1 lectin dylight
( A ) A phage-displayed random peptide library was used to select phages that bind to the NSCLC cell line CL1-5. ( B ) Visualization of PC5-2 binding to CL1-5 and PC13 lung cancer cells (arrowheads) with immunohistochemical staining. The control phage did not bind to CL1-5 cells. Scale bar: 10 µm. ( C ) The FITC-labeled peptide SP5-2 bound to five NSCLC cell lines but not to NPC-TW01 cells as detected by immunofluorescent staining. Scale bar: 10 µm. ( D ) Representative photomicrographs of tumor sections from surgical specimens of human lung cancer were detected using both PC5-2 (a, arrowhead) and <t>biotinylated</t> SP5-2 (c, arrowhead), respectively. In comparison, the control phage or biotinylated control peptide could not bind to these surgical specimens (b and d). PC5-2 was competitively inhibited by the synthetic peptide SP5-2 (e). Mutated peptide, MP5-2, lost this competition ability (f). Scale bar: 25 µm.
Ihc Vector Labs Dl 1178 1 Lectin Dylight, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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galectin-3  (Galectin Therapeutics)
90
Galectin Therapeutics galectin-3
( A ) A phage-displayed random peptide library was used to select phages that bind to the NSCLC cell line CL1-5. ( B ) Visualization of PC5-2 binding to CL1-5 and PC13 lung cancer cells (arrowheads) with immunohistochemical staining. The control phage did not bind to CL1-5 cells. Scale bar: 10 µm. ( C ) The FITC-labeled peptide SP5-2 bound to five NSCLC cell lines but not to NPC-TW01 cells as detected by immunofluorescent staining. Scale bar: 10 µm. ( D ) Representative photomicrographs of tumor sections from surgical specimens of human lung cancer were detected using both PC5-2 (a, arrowhead) and <t>biotinylated</t> SP5-2 (c, arrowhead), respectively. In comparison, the control phage or biotinylated control peptide could not bind to these surgical specimens (b and d). PC5-2 was competitively inhibited by the synthetic peptide SP5-2 (e). Mutated peptide, MP5-2, lost this competition ability (f). Scale bar: 25 µm.
Galectin 3, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse biotinylated gs ib4  (Vector Laboratories)
96
Vector Laboratories mouse biotinylated gs ib4
( A ) A phage-displayed random peptide library was used to select phages that bind to the NSCLC cell line CL1-5. ( B ) Visualization of PC5-2 binding to CL1-5 and PC13 lung cancer cells (arrowheads) with immunohistochemical staining. The control phage did not bind to CL1-5 cells. Scale bar: 10 µm. ( C ) The FITC-labeled peptide SP5-2 bound to five NSCLC cell lines but not to NPC-TW01 cells as detected by immunofluorescent staining. Scale bar: 10 µm. ( D ) Representative photomicrographs of tumor sections from surgical specimens of human lung cancer were detected using both PC5-2 (a, arrowhead) and <t>biotinylated</t> SP5-2 (c, arrowhead), respectively. In comparison, the control phage or biotinylated control peptide could not bind to these surgical specimens (b and d). PC5-2 was competitively inhibited by the synthetic peptide SP5-2 (e). Mutated peptide, MP5-2, lost this competition ability (f). Scale bar: 25 µm.
Mouse Biotinylated Gs Ib4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rhodamine peanut agglutinin pna conjugate 566  (Vector Laboratories)
95
Vector Laboratories rhodamine peanut agglutinin pna conjugate 566
( A ) A phage-displayed random peptide library was used to select phages that bind to the NSCLC cell line CL1-5. ( B ) Visualization of PC5-2 binding to CL1-5 and PC13 lung cancer cells (arrowheads) with immunohistochemical staining. The control phage did not bind to CL1-5 cells. Scale bar: 10 µm. ( C ) The FITC-labeled peptide SP5-2 bound to five NSCLC cell lines but not to NPC-TW01 cells as detected by immunofluorescent staining. Scale bar: 10 µm. ( D ) Representative photomicrographs of tumor sections from surgical specimens of human lung cancer were detected using both PC5-2 (a, arrowhead) and <t>biotinylated</t> SP5-2 (c, arrowhead), respectively. In comparison, the control phage or biotinylated control peptide could not bind to these surgical specimens (b and d). PC5-2 was competitively inhibited by the synthetic peptide SP5-2 (e). Mutated peptide, MP5-2, lost this competition ability (f). Scale bar: 25 µm.
Rhodamine Peanut Agglutinin Pna Conjugate 566, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit ficolin  (Bioss)
92
Bioss rabbit ficolin
Expression levels of 17 genes based on GEPIA2 (Gene Expression Profiling Interactive Analysis web server). (A) ANGPTL6 . (B) CFP . (C) CLEC1B . (D) CLEC4G . (E) CLEC4M . (F) COLEC10 . (G) CRHBP . (H) CXCL12 . (I) DNASE1L3 . (J) <t>FCN2</t> . (K) FCN3 . (L) GSTZ1 . (M) LCAT . (N) NAT2 . (O) OIT3 . (P) RSPO3 . (Q) VIPR1 . * p < 0.05. The y -axis represents the relative log2 expression value (TPM + 1).
Rabbit Ficolin, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-cd68  (Danaher Inc)
90
Danaher Inc anti-cd68
Expression levels of 17 genes based on GEPIA2 (Gene Expression Profiling Interactive Analysis web server). (A) ANGPTL6 . (B) CFP . (C) CLEC1B . (D) CLEC4G . (E) CLEC4M . (F) COLEC10 . (G) CRHBP . (H) CXCL12 . (I) DNASE1L3 . (J) <t>FCN2</t> . (K) FCN3 . (L) GSTZ1 . (M) LCAT . (N) NAT2 . (O) OIT3 . (P) RSPO3 . (Q) VIPR1 . * p < 0.05. The y -axis represents the relative log2 expression value (TPM + 1).
Anti Cd68, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lotus tetragonolobus lectin biotin conjugated  (Vector Laboratories)
96
Vector Laboratories lotus tetragonolobus lectin biotin conjugated
(a) Immunocytochemistry for SIX2 at day 9 of differentiation revealing NPCs. Scale bar: 50 μm. (b) Immunocytochemistry to identify nephron segments in 2D culture on day 21 of the differentiation. Scale bar: 50 μm. (c) Immunohistochemistry to identify nephron segments in 3D culture with frozen sections on day 21 of differentiation. Scale bar: 50 μm. (d) Whole mount staining for nephrons in 3D culture on day 28 (left: high magnification, scale bar: 50 μm) and 21 (right: low magnification, scale bar: 100 μm). The inset on the right shows DAPI (4′,6-diamidino-2-phenylindole) staining. PODXL: podocalyxin (a podocyte marker). LTL: lotus <t>tetragonolobus</t> <t>lectin</t> (a proximal tubule marker). CDH1: cadherin1 (also known as E-cadherin) (a loop of Henle and distal tubule marker). (e) Bright field imaging of an organoid in 3D culture on day 21. Arrows indicate a glomerular structure. Scale bar: 100 μm. (c) and the left panel of (d) were originally published in ref 15.
Lotus Tetragonolobus Lectin Biotin Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ihc  (Galectin Therapeutics)
90
Galectin Therapeutics ihc
(a) Immunocytochemistry for SIX2 at day 9 of differentiation revealing NPCs. Scale bar: 50 μm. (b) Immunocytochemistry to identify nephron segments in 2D culture on day 21 of the differentiation. Scale bar: 50 μm. (c) Immunohistochemistry to identify nephron segments in 3D culture with frozen sections on day 21 of differentiation. Scale bar: 50 μm. (d) Whole mount staining for nephrons in 3D culture on day 28 (left: high magnification, scale bar: 50 μm) and 21 (right: low magnification, scale bar: 100 μm). The inset on the right shows DAPI (4′,6-diamidino-2-phenylindole) staining. PODXL: podocalyxin (a podocyte marker). LTL: lotus <t>tetragonolobus</t> <t>lectin</t> (a proximal tubule marker). CDH1: cadherin1 (also known as E-cadherin) (a loop of Henle and distal tubule marker). (e) Bright field imaging of an organoid in 3D culture on day 21. Arrows indicate a glomerular structure. Scale bar: 100 μm. (c) and the left panel of (d) were originally published in ref 15.
Ihc, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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purpurea lectin  (Vector Laboratories)
92
Vector Laboratories purpurea lectin
Deficiency of centroacinar cells (CACs) in Gfi1 -null pancreas. ( A, B ) Electron micrographs of WT and Gfi1 -null pancreas. In the WT pancreas, a single CAC ( red dotted line ) is surrounded by zymogen (z.g.)-rich acinar cells, with which they share a lumen (Lu) ( A ). In contrast to the WT pancreas, in the Gfi1 -null pancreas the structure consisting of CACs immediately adjacent to the exocrine apical surface was defective ( B ). ( C–F ) Immunohistochemical analysis of Bauhenia <t>purpurea</t> <t>lectin</t> (BPL). BPL reactivity was completely absent in the E18.5 Gfi1 -null pancreas ( D ) compared with WT ( C ). BPL reactivity was disorganized and observed with the distorted acinar units in the 1-month-old (1M) Gfi1 -null pancreas (F). ( G, H ) Immunohistochemical analysis of Claudin10. The staining of Claudin10 was diminished at the apical membrane of pancreatic exocrine cells in Gfi1 -nulls ( H ) when compared with WT ( G ). ( I–N ) Immunohistochemical analysis of Mucin1 (Muc1). In the E18.5 WT pancreas, Mucin1 antibodies labeled all cells of the ductal system, including intercalated ducts and CACs, showing the well-structured branching of the ductal system and the radiations of small luminal areas ( I ). The Gfi1 -null pancreas contained intercalated ductal cells but showed a lack of the terminal, radiating, branching structures observed in WT ( J ). The phenotype was more pronounced at 1 month old (1M) ( L ) compared with WT littermates ( K ). Progression and worsening of acinar structural defects continued over time; at 4 months old (4M), Muc1-positive areas outline the extensive vacuoles observed in the Gfi1 -null pancreas ( N ) compared with WT ( M ). ( O, P ) At the postnatal stage (6 days after birth, P6), immunochemical analysis of Claudin10 and Sox9 demonstrates the defects in acinar apical membranes and CACs in Gfi1 -null mice. In the WT pancreas, Claudin10 protein ( arrows ) specifically localizes at the apical membranes of acinar cells, where the duct cells including Sox9 + CACs ( arrowheads ) interconnect with acinar cells ( O ). However, in Gfi1 -null mice, Claudin10 expression at apical membranes of acinar cells ( arrows ) dramatically decreased, and Sox9 + CACs ( arrowheads ) were rarely found ( P ). Scale bar: 50 μm.
Purpurea Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated ricinus communis agglutinin rca  (Vector Laboratories)
95
Vector Laboratories biotinylated ricinus communis agglutinin rca
Parabiotic demonstration of blood-borne CD34+ endothelial cells on Day 7 after pneumonectomy. (A) Schematic of experimental design involving a left pneumonectomy in the wild-type parabiont of wild-type/GFP+ parabiotic twins (C57/B6). Parabiosis was established for 28 days prior to left pneumonectomy. The remaining lung was studied 7 days after pneumonectomy. (B) <t>Anti-GFP</t> <t>avidin–biotin</t> complex (ABC) immunohistochemical staining demonstrated GFP+ cells within the alveolar septae. Flow cytometry of the lung digests demonstrated GFP+ cells that were CD45− (C) and CD31+ (D). (E and F) Analysis of CD31+ lung endothelial cells demonstrated that most of the GFP expression occurred in the CD34+ endothelial cell population (P < 0.01; n = 5).
Biotinylated Ricinus Communis Agglutinin Rca, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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collagen iii  (Proteintech)
95
Proteintech collagen iii
<t>Collagen</t> I, collagen <t>III,</t> and TGF-β expression in the cardiac tissues of three groups of rats fed different diets. (A) Representative immunohistochemical staining for collagen I, collagen III, and TGF-β. Arrows indicate positively stained cells. (B) Bar graph showing collagen I, collagen III, and TGF-β positive cells. Data are presented as the mean ± SEM; n = 3 in each group. * P < 0.05 vs HFD group. (C) Immunoblotting for collagen I, collagen III, and TGF-β protein expression in cardiac tissues. (D) The bar graph shows the quantification of collagen I, collagen III, and TGF-β protein expression. Data are presented as the mean ± SEM; n = 3 in each group. * P < 0.05 vs HFD group.
Collagen Iii, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) A phage-displayed random peptide library was used to select phages that bind to the NSCLC cell line CL1-5. ( B ) Visualization of PC5-2 binding to CL1-5 and PC13 lung cancer cells (arrowheads) with immunohistochemical staining. The control phage did not bind to CL1-5 cells. Scale bar: 10 µm. ( C ) The FITC-labeled peptide SP5-2 bound to five NSCLC cell lines but not to NPC-TW01 cells as detected by immunofluorescent staining. Scale bar: 10 µm. ( D ) Representative photomicrographs of tumor sections from surgical specimens of human lung cancer were detected using both PC5-2 (a, arrowhead) and biotinylated SP5-2 (c, arrowhead), respectively. In comparison, the control phage or biotinylated control peptide could not bind to these surgical specimens (b and d). PC5-2 was competitively inhibited by the synthetic peptide SP5-2 (e). Mutated peptide, MP5-2, lost this competition ability (f). Scale bar: 25 µm.

Journal: PLoS ONE

Article Title: A Novel Peptide Enhances Therapeutic Efficacy of Liposomal Anti-Cancer Drugs in Mice Models of Human Lung Cancer

doi: 10.1371/journal.pone.0004171

Figure Lengend Snippet: ( A ) A phage-displayed random peptide library was used to select phages that bind to the NSCLC cell line CL1-5. ( B ) Visualization of PC5-2 binding to CL1-5 and PC13 lung cancer cells (arrowheads) with immunohistochemical staining. The control phage did not bind to CL1-5 cells. Scale bar: 10 µm. ( C ) The FITC-labeled peptide SP5-2 bound to five NSCLC cell lines but not to NPC-TW01 cells as detected by immunofluorescent staining. Scale bar: 10 µm. ( D ) Representative photomicrographs of tumor sections from surgical specimens of human lung cancer were detected using both PC5-2 (a, arrowhead) and biotinylated SP5-2 (c, arrowhead), respectively. In comparison, the control phage or biotinylated control peptide could not bind to these surgical specimens (b and d). PC5-2 was competitively inhibited by the synthetic peptide SP5-2 (e). Mutated peptide, MP5-2, lost this competition ability (f). Scale bar: 25 µm.

Article Snippet: The biotinylated lectin was visualized with streptavidin-conjugated rhodamine (Pierce, IL, USA).

Techniques: Binding Assay, Immunohistochemical staining, Staining, Control, Labeling, Comparison

( A ) Representative two-color images showing the distribution of doxorubicin (red) in relation to nuclei (blue) in tissue sections. Accumulation of doxorubicin in tumor tissues was shown at 1, 4 and 24 hours post-injection. Bar, 50 µm. ( B ) Histopathology and fluorescent staining of tumor tissues in each treatment group examined after staining with H&E, TUNEL (green), lectin (red), and DAPI (blue). Bar, 50 µm. Enhancement of drug accumulation in tumor tissues correlated with the increased therapeutic efficacy.

Journal: PLoS ONE

Article Title: A Novel Peptide Enhances Therapeutic Efficacy of Liposomal Anti-Cancer Drugs in Mice Models of Human Lung Cancer

doi: 10.1371/journal.pone.0004171

Figure Lengend Snippet: ( A ) Representative two-color images showing the distribution of doxorubicin (red) in relation to nuclei (blue) in tissue sections. Accumulation of doxorubicin in tumor tissues was shown at 1, 4 and 24 hours post-injection. Bar, 50 µm. ( B ) Histopathology and fluorescent staining of tumor tissues in each treatment group examined after staining with H&E, TUNEL (green), lectin (red), and DAPI (blue). Bar, 50 µm. Enhancement of drug accumulation in tumor tissues correlated with the increased therapeutic efficacy.

Article Snippet: The biotinylated lectin was visualized with streptavidin-conjugated rhodamine (Pierce, IL, USA).

Techniques: Injection, Histopathology, Staining, TUNEL Assay, Drug discovery

Expression levels of 17 genes based on GEPIA2 (Gene Expression Profiling Interactive Analysis web server). (A) ANGPTL6 . (B) CFP . (C) CLEC1B . (D) CLEC4G . (E) CLEC4M . (F) COLEC10 . (G) CRHBP . (H) CXCL12 . (I) DNASE1L3 . (J) FCN2 . (K) FCN3 . (L) GSTZ1 . (M) LCAT . (N) NAT2 . (O) OIT3 . (P) RSPO3 . (Q) VIPR1 . * p < 0.05. The y -axis represents the relative log2 expression value (TPM + 1).

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Expression levels of 17 genes based on GEPIA2 (Gene Expression Profiling Interactive Analysis web server). (A) ANGPTL6 . (B) CFP . (C) CLEC1B . (D) CLEC4G . (E) CLEC4M . (F) COLEC10 . (G) CRHBP . (H) CXCL12 . (I) DNASE1L3 . (J) FCN2 . (K) FCN3 . (L) GSTZ1 . (M) LCAT . (N) NAT2 . (O) OIT3 . (P) RSPO3 . (Q) VIPR1 . * p < 0.05. The y -axis represents the relative log2 expression value (TPM + 1).

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Expressing

Survival analysis of 17 genes. (A) ANGPTL6 . (B) CFP . (C) CLEC1B . (D) CLEC4G . (E) CLEC4M . (F) COLEC10 . (G) CRHBP . (H) CXCL12 . (I) DNASE1L3 . (J) FCN2 . (K) FCN3 . (L) GSTZ1 . (M) LCAT . (N) NAT2 . (O) OIT3 . (P) RSPO3 . (Q) VIPR1 . (R) Constructed protein–protein interaction (PPI) network of the important differentially expressed genes (DEGs) using STRING. (S) Use of the Cytoscape plug-in MCODE to select the most important module from the PPI network.

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Survival analysis of 17 genes. (A) ANGPTL6 . (B) CFP . (C) CLEC1B . (D) CLEC4G . (E) CLEC4M . (F) COLEC10 . (G) CRHBP . (H) CXCL12 . (I) DNASE1L3 . (J) FCN2 . (K) FCN3 . (L) GSTZ1 . (M) LCAT . (N) NAT2 . (O) OIT3 . (P) RSPO3 . (Q) VIPR1 . (R) Constructed protein–protein interaction (PPI) network of the important differentially expressed genes (DEGs) using STRING. (S) Use of the Cytoscape plug-in MCODE to select the most important module from the PPI network.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Construct

Pan-tissue expression of FCN2 . (A) Pan-cancer expression of FCN2 in the Oncomine database. (B) Expression of FCN2 in normal tissues in the BioGPS database. (C) Pan-cancer expression of FCN2 in the UCSC (University of California, Santa Cruz) database. (D) Log2 transformation of the pan-cancer expression value of FCN2 in the UCSC database. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not statistically significant.

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Pan-tissue expression of FCN2 . (A) Pan-cancer expression of FCN2 in the Oncomine database. (B) Expression of FCN2 in normal tissues in the BioGPS database. (C) Pan-cancer expression of FCN2 in the UCSC (University of California, Santa Cruz) database. (D) Log2 transformation of the pan-cancer expression value of FCN2 in the UCSC database. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not statistically significant.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Expressing, Transformation Assay

Transcription levels of FCN2 in liver cancer and normal tissues. (A–C) Expression differences of the FCN2 gene between liver cancer tissues and normal tissues in the Oncomine database. (D) FCN2 mRNA expression in liver cancer tissue and normal tissue based on the GEPIA2 database (TCGA tumors vs . TCGA normal). (E, F) FCN2 protein expression in hepatocellular carcinoma in the UALCAN database. (G) Part of the results of the immunohistochemistry experiments. (H) Average optical density of liver cancer tissues and adjacent tissues from 30 liver cancer patients. TCGA , The Cancer Genome Atlas. * p < 0.05, *** p < 0.001.

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Transcription levels of FCN2 in liver cancer and normal tissues. (A–C) Expression differences of the FCN2 gene between liver cancer tissues and normal tissues in the Oncomine database. (D) FCN2 mRNA expression in liver cancer tissue and normal tissue based on the GEPIA2 database (TCGA tumors vs . TCGA normal). (E, F) FCN2 protein expression in hepatocellular carcinoma in the UALCAN database. (G) Part of the results of the immunohistochemistry experiments. (H) Average optical density of liver cancer tissues and adjacent tissues from 30 liver cancer patients. TCGA , The Cancer Genome Atlas. * p < 0.05, *** p < 0.001.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Expressing, Immunohistochemistry

Different expression levels of FCN2 in liver hepatocellular carcinoma (LIHC) based on The Cancer Genome Atlas (TCGA) analyzed using R. (A) Pathologic stages. (B) Histological grade. (C) Fibrosis Ishak scale scores. (D) Race. (E) Normal tissue and liver cancer. (F) Adjacent hepatic tissue inflammation. (G) Age (H) . BMI. (I) Alpha-fetoprotein (AFP). (J) Vascular invasion. (K) Gender. * p < 0.05, ** p < 0.01, *** p < 0.001. ns: not statistically significant.

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Different expression levels of FCN2 in liver hepatocellular carcinoma (LIHC) based on The Cancer Genome Atlas (TCGA) analyzed using R. (A) Pathologic stages. (B) Histological grade. (C) Fibrosis Ishak scale scores. (D) Race. (E) Normal tissue and liver cancer. (F) Adjacent hepatic tissue inflammation. (G) Age (H) . BMI. (I) Alpha-fetoprotein (AFP). (J) Vascular invasion. (K) Gender. * p < 0.05, ** p < 0.01, *** p < 0.001. ns: not statistically significant.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Expressing

Prognostic value of FCN2 in liver cancer. (A–D) Overall survival (OS) (A) , relapse-free survival (RFS) (B) , disease-specific survival (DSS) (C) , and patient-free survival/progression-free survival (PFS) (D) . (E) Nomogram predicting the 1-, 3-, and 5-year OS probability. (F) C-index of the prognostic model nomogram for predicting the 1-, 3-, and 5-year OS probability in hepatocellular carcinoma (HCC) patients. (G) Prediction of the 1-, 3-, and 5-year OS probability using nomogram calibration plots.

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Prognostic value of FCN2 in liver cancer. (A–D) Overall survival (OS) (A) , relapse-free survival (RFS) (B) , disease-specific survival (DSS) (C) , and patient-free survival/progression-free survival (PFS) (D) . (E) Nomogram predicting the 1-, 3-, and 5-year OS probability. (F) C-index of the prognostic model nomogram for predicting the 1-, 3-, and 5-year OS probability in hepatocellular carcinoma (HCC) patients. (G) Prediction of the 1-, 3-, and 5-year OS probability using nomogram calibration plots.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques:

Protein–protein interaction network analysis and enrichment analysis. (A, B) Gene network diagram of interaction with FCN2 created using the STRING database. (C–G) Diagrams of the analyses of the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) pathways.

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Protein–protein interaction network analysis and enrichment analysis. (A, B) Gene network diagram of interaction with FCN2 created using the STRING database. (C–G) Diagrams of the analyses of the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) pathways.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques:

Correlation of FCN2 expression with immune characteristics. (A) Differential distribution of the immune cells in patients with high and low FCN2 expressions. (B–G) Correlation between the expression level of FCN2 and immune infiltration in hepatocellular carcinoma: (B) Neutrophils, (C) Eosinophils, (D) NK cells, (E) Tcm, (F) DC, (G) Th2 cells. * p < 0.05, ** p < 0.01, *** p < 0.001. ns, no significance.

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Correlation of FCN2 expression with immune characteristics. (A) Differential distribution of the immune cells in patients with high and low FCN2 expressions. (B–G) Correlation between the expression level of FCN2 and immune infiltration in hepatocellular carcinoma: (B) Neutrophils, (C) Eosinophils, (D) NK cells, (E) Tcm, (F) DC, (G) Th2 cells. * p < 0.05, ** p < 0.01, *** p < 0.001. ns, no significance.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Expressing

Correlation analysis between FCN2 and the biomarkers of immune cells in hepatocellular carcinoma (HCC) determined using the GEPIA database (Spearman’s correlation coefficient).

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Correlation analysis between FCN2 and the biomarkers of immune cells in hepatocellular carcinoma (HCC) determined using the GEPIA database (Spearman’s correlation coefficient).

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques:

Immunomodulators, chemokines, and receptors associated with FCN2 . (A) Distribution of the FCN2 immunological scores in tumor and normal tissues.The ordinate reflects the distribution of the immunological scores in distinct groups, whereas the abscissa indicates the immune cell types. The Wilcoxon test was used to compare statistical differences between the two groups, and the Kruskal–Wallis test was used to determine the significance of the differences between three groups. ( a ) Heatmap of the immune cell scores. Different hues represent the varied expression distributions in different samples. Asterisks indicate significance levels at * p < 0.05, ** p < 0.01, and *** p < 0.001. ( b ) Percentages of tumor-infiltrating immune cells in each sample. Different colors depict the different types of immunological cells. The abscissa denotes the sample, whereas the ordinate denotes the percentage of immune cells in a single sample. (H) Immunomodulators, chemokines, and receptors associated with FCN2 in liver hepatocellular carcinoma (LIHC). (B) CCL14. (C) CCL16. (D) CCL23. (E) TNFSF4. (F) CD276. (G) TNFRSF4. (H) KDR. **** p < 0.0001.

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Immunomodulators, chemokines, and receptors associated with FCN2 . (A) Distribution of the FCN2 immunological scores in tumor and normal tissues.The ordinate reflects the distribution of the immunological scores in distinct groups, whereas the abscissa indicates the immune cell types. The Wilcoxon test was used to compare statistical differences between the two groups, and the Kruskal–Wallis test was used to determine the significance of the differences between three groups. ( a ) Heatmap of the immune cell scores. Different hues represent the varied expression distributions in different samples. Asterisks indicate significance levels at * p < 0.05, ** p < 0.01, and *** p < 0.001. ( b ) Percentages of tumor-infiltrating immune cells in each sample. Different colors depict the different types of immunological cells. The abscissa denotes the sample, whereas the ordinate denotes the percentage of immune cells in a single sample. (H) Immunomodulators, chemokines, and receptors associated with FCN2 in liver hepatocellular carcinoma (LIHC). (B) CCL14. (C) CCL16. (D) CCL23. (E) TNFSF4. (F) CD276. (G) TNFRSF4. (H) KDR. **** p < 0.0001.

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Expressing

Immunohistochemical comparison of FCN2 and the molecules with strong correlations [based on the Human Protein Atlas (HPA)].

Journal: Frontiers in Oncology

Article Title: Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer

doi: 10.3389/fonc.2022.987481

Figure Lengend Snippet: Immunohistochemical comparison of FCN2 and the molecules with strong correlations [based on the Human Protein Atlas (HPA)].

Article Snippet: The samples were paraffin-fixed, cut into serial sections, and incubated with rabbit ficolin-2/ficolin-B polyclonal antibody (bs-13162R; Bioss, Woburn, MA, USA) at 4°C overnight.

Techniques: Immunohistochemical staining

(a) Immunocytochemistry for SIX2 at day 9 of differentiation revealing NPCs. Scale bar: 50 μm. (b) Immunocytochemistry to identify nephron segments in 2D culture on day 21 of the differentiation. Scale bar: 50 μm. (c) Immunohistochemistry to identify nephron segments in 3D culture with frozen sections on day 21 of differentiation. Scale bar: 50 μm. (d) Whole mount staining for nephrons in 3D culture on day 28 (left: high magnification, scale bar: 50 μm) and 21 (right: low magnification, scale bar: 100 μm). The inset on the right shows DAPI (4′,6-diamidino-2-phenylindole) staining. PODXL: podocalyxin (a podocyte marker). LTL: lotus tetragonolobus lectin (a proximal tubule marker). CDH1: cadherin1 (also known as E-cadherin) (a loop of Henle and distal tubule marker). (e) Bright field imaging of an organoid in 3D culture on day 21. Arrows indicate a glomerular structure. Scale bar: 100 μm. (c) and the left panel of (d) were originally published in ref 15.

Journal: Nature protocols

Article Title: Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells

doi: 10.1038/nprot.2016.170

Figure Lengend Snippet: (a) Immunocytochemistry for SIX2 at day 9 of differentiation revealing NPCs. Scale bar: 50 μm. (b) Immunocytochemistry to identify nephron segments in 2D culture on day 21 of the differentiation. Scale bar: 50 μm. (c) Immunohistochemistry to identify nephron segments in 3D culture with frozen sections on day 21 of differentiation. Scale bar: 50 μm. (d) Whole mount staining for nephrons in 3D culture on day 28 (left: high magnification, scale bar: 50 μm) and 21 (right: low magnification, scale bar: 100 μm). The inset on the right shows DAPI (4′,6-diamidino-2-phenylindole) staining. PODXL: podocalyxin (a podocyte marker). LTL: lotus tetragonolobus lectin (a proximal tubule marker). CDH1: cadherin1 (also known as E-cadherin) (a loop of Henle and distal tubule marker). (e) Bright field imaging of an organoid in 3D culture on day 21. Arrows indicate a glomerular structure. Scale bar: 100 μm. (c) and the left panel of (d) were originally published in ref 15.

Article Snippet: Incubate the plate overnight at 4 °C. table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Host Supplier Cat. No. Dilution α-SMA Mouse Sigma F3777 1:500 Aquaporin2 Rabbit Millipore AB3274 1:100 E-cadherin (CDH1) Rat Abcam ab11512 1:500 Endomucin Rat Abcam ab45771 1:500 HOXD11 Mouse Sigma SAB1403944 1:500 KIM1 Goat R&D AF1750 1:500 Laminin Rabbit Sigma L9393 1:500 LHX1 Mouse Developmental Studies Hybridoma Bank 4F2-c 1:50 LTL (lotus tetragonolobus lectin) Biotin conjugated Vector lab B-1325 1:200 PAX8 Rabbit Proteintech 10336-1-AP 1:500 PDGFR-b Goat Novus AF385 1:100 Podocalyxin (PODXL) Goat R&D systems AF1658 1:500 SIX2 Rabbit Proteintech 11562-1-AP 1:500 Uromodulin Rabbit Biomedical Technologies BT-590 1:150 WT-1 Rabbit Santa cruz sc-192 1:50 Open in a separate window Antibody list CRITICAL STEP If the surface of the wells is not covered fully by the antibody dilution buffer, increase the antibody solution amount from 150 to 200 μl.

Techniques: Immunocytochemistry, Immunohistochemistry, Staining, Marker, Imaging

Immunohistochemical staining for CDH1 (cadherin1), KIM1 (kidney injury molecule1), and LTL (lotus tetragonolobus lectin) in kidney organoids after 24 hours treatment with cisplatin 5 μM. LTL+ tubules expressed KIM1 after the treatment, which is a marker for proximal tubular injury. Kidney organoids generated in 3D culture were treated with cisplatin 5 μM for 24 hours from day 23 to 24 of the differentiation. Organoids were fixed and analyzed on day 24. Scale bars: 50 μm. The scale bars are representative of the corresponding right panels.

Journal: Nature protocols

Article Title: Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells

doi: 10.1038/nprot.2016.170

Figure Lengend Snippet: Immunohistochemical staining for CDH1 (cadherin1), KIM1 (kidney injury molecule1), and LTL (lotus tetragonolobus lectin) in kidney organoids after 24 hours treatment with cisplatin 5 μM. LTL+ tubules expressed KIM1 after the treatment, which is a marker for proximal tubular injury. Kidney organoids generated in 3D culture were treated with cisplatin 5 μM for 24 hours from day 23 to 24 of the differentiation. Organoids were fixed and analyzed on day 24. Scale bars: 50 μm. The scale bars are representative of the corresponding right panels.

Article Snippet: Incubate the plate overnight at 4 °C. table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Host Supplier Cat. No. Dilution α-SMA Mouse Sigma F3777 1:500 Aquaporin2 Rabbit Millipore AB3274 1:100 E-cadherin (CDH1) Rat Abcam ab11512 1:500 Endomucin Rat Abcam ab45771 1:500 HOXD11 Mouse Sigma SAB1403944 1:500 KIM1 Goat R&D AF1750 1:500 Laminin Rabbit Sigma L9393 1:500 LHX1 Mouse Developmental Studies Hybridoma Bank 4F2-c 1:50 LTL (lotus tetragonolobus lectin) Biotin conjugated Vector lab B-1325 1:200 PAX8 Rabbit Proteintech 10336-1-AP 1:500 PDGFR-b Goat Novus AF385 1:100 Podocalyxin (PODXL) Goat R&D systems AF1658 1:500 SIX2 Rabbit Proteintech 11562-1-AP 1:500 Uromodulin Rabbit Biomedical Technologies BT-590 1:150 WT-1 Rabbit Santa cruz sc-192 1:50 Open in a separate window Antibody list CRITICAL STEP If the surface of the wells is not covered fully by the antibody dilution buffer, increase the antibody solution amount from 150 to 200 μl.

Techniques: Immunohistochemical staining, Staining, Marker, Generated

Antibody list

Journal: Nature protocols

Article Title: Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells

doi: 10.1038/nprot.2016.170

Figure Lengend Snippet: Antibody list

Article Snippet: Incubate the plate overnight at 4 °C. table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Host Supplier Cat. No. Dilution α-SMA Mouse Sigma F3777 1:500 Aquaporin2 Rabbit Millipore AB3274 1:100 E-cadherin (CDH1) Rat Abcam ab11512 1:500 Endomucin Rat Abcam ab45771 1:500 HOXD11 Mouse Sigma SAB1403944 1:500 KIM1 Goat R&D AF1750 1:500 Laminin Rabbit Sigma L9393 1:500 LHX1 Mouse Developmental Studies Hybridoma Bank 4F2-c 1:50 LTL (lotus tetragonolobus lectin) Biotin conjugated Vector lab B-1325 1:200 PAX8 Rabbit Proteintech 10336-1-AP 1:500 PDGFR-b Goat Novus AF385 1:100 Podocalyxin (PODXL) Goat R&D systems AF1658 1:500 SIX2 Rabbit Proteintech 11562-1-AP 1:500 Uromodulin Rabbit Biomedical Technologies BT-590 1:150 WT-1 Rabbit Santa cruz sc-192 1:50 Open in a separate window Antibody list CRITICAL STEP If the surface of the wells is not covered fully by the antibody dilution buffer, increase the antibody solution amount from 150 to 200 μl.

Techniques: Plasmid Preparation

Deficiency of centroacinar cells (CACs) in Gfi1 -null pancreas. ( A, B ) Electron micrographs of WT and Gfi1 -null pancreas. In the WT pancreas, a single CAC ( red dotted line ) is surrounded by zymogen (z.g.)-rich acinar cells, with which they share a lumen (Lu) ( A ). In contrast to the WT pancreas, in the Gfi1 -null pancreas the structure consisting of CACs immediately adjacent to the exocrine apical surface was defective ( B ). ( C–F ) Immunohistochemical analysis of Bauhenia purpurea lectin (BPL). BPL reactivity was completely absent in the E18.5 Gfi1 -null pancreas ( D ) compared with WT ( C ). BPL reactivity was disorganized and observed with the distorted acinar units in the 1-month-old (1M) Gfi1 -null pancreas (F). ( G, H ) Immunohistochemical analysis of Claudin10. The staining of Claudin10 was diminished at the apical membrane of pancreatic exocrine cells in Gfi1 -nulls ( H ) when compared with WT ( G ). ( I–N ) Immunohistochemical analysis of Mucin1 (Muc1). In the E18.5 WT pancreas, Mucin1 antibodies labeled all cells of the ductal system, including intercalated ducts and CACs, showing the well-structured branching of the ductal system and the radiations of small luminal areas ( I ). The Gfi1 -null pancreas contained intercalated ductal cells but showed a lack of the terminal, radiating, branching structures observed in WT ( J ). The phenotype was more pronounced at 1 month old (1M) ( L ) compared with WT littermates ( K ). Progression and worsening of acinar structural defects continued over time; at 4 months old (4M), Muc1-positive areas outline the extensive vacuoles observed in the Gfi1 -null pancreas ( N ) compared with WT ( M ). ( O, P ) At the postnatal stage (6 days after birth, P6), immunochemical analysis of Claudin10 and Sox9 demonstrates the defects in acinar apical membranes and CACs in Gfi1 -null mice. In the WT pancreas, Claudin10 protein ( arrows ) specifically localizes at the apical membranes of acinar cells, where the duct cells including Sox9 + CACs ( arrowheads ) interconnect with acinar cells ( O ). However, in Gfi1 -null mice, Claudin10 expression at apical membranes of acinar cells ( arrows ) dramatically decreased, and Sox9 + CACs ( arrowheads ) were rarely found ( P ). Scale bar: 50 μm.

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Growth Factor Independence-1 ( Gfi1 ) Is Required for Pancreatic Acinar Unit Formation and Centroacinar Cell Differentiation

doi: 10.1016/j.jcmgh.2014.12.004

Figure Lengend Snippet: Deficiency of centroacinar cells (CACs) in Gfi1 -null pancreas. ( A, B ) Electron micrographs of WT and Gfi1 -null pancreas. In the WT pancreas, a single CAC ( red dotted line ) is surrounded by zymogen (z.g.)-rich acinar cells, with which they share a lumen (Lu) ( A ). In contrast to the WT pancreas, in the Gfi1 -null pancreas the structure consisting of CACs immediately adjacent to the exocrine apical surface was defective ( B ). ( C–F ) Immunohistochemical analysis of Bauhenia purpurea lectin (BPL). BPL reactivity was completely absent in the E18.5 Gfi1 -null pancreas ( D ) compared with WT ( C ). BPL reactivity was disorganized and observed with the distorted acinar units in the 1-month-old (1M) Gfi1 -null pancreas (F). ( G, H ) Immunohistochemical analysis of Claudin10. The staining of Claudin10 was diminished at the apical membrane of pancreatic exocrine cells in Gfi1 -nulls ( H ) when compared with WT ( G ). ( I–N ) Immunohistochemical analysis of Mucin1 (Muc1). In the E18.5 WT pancreas, Mucin1 antibodies labeled all cells of the ductal system, including intercalated ducts and CACs, showing the well-structured branching of the ductal system and the radiations of small luminal areas ( I ). The Gfi1 -null pancreas contained intercalated ductal cells but showed a lack of the terminal, radiating, branching structures observed in WT ( J ). The phenotype was more pronounced at 1 month old (1M) ( L ) compared with WT littermates ( K ). Progression and worsening of acinar structural defects continued over time; at 4 months old (4M), Muc1-positive areas outline the extensive vacuoles observed in the Gfi1 -null pancreas ( N ) compared with WT ( M ). ( O, P ) At the postnatal stage (6 days after birth, P6), immunochemical analysis of Claudin10 and Sox9 demonstrates the defects in acinar apical membranes and CACs in Gfi1 -null mice. In the WT pancreas, Claudin10 protein ( arrows ) specifically localizes at the apical membranes of acinar cells, where the duct cells including Sox9 + CACs ( arrowheads ) interconnect with acinar cells ( O ). However, in Gfi1 -null mice, Claudin10 expression at apical membranes of acinar cells ( arrows ) dramatically decreased, and Sox9 + CACs ( arrowheads ) were rarely found ( P ). Scale bar: 50 μm.

Article Snippet: Fluorescein-Bauhenia , Purpurea lectin , Vector Labs, Burlingame, CA , FL-1281 , Q0910 , 1:100.

Techniques: Immunohistochemical staining, Staining, Labeling, Expressing

Information about Primary Antibodies and Lectin Dye

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Growth Factor Independence-1 ( Gfi1 ) Is Required for Pancreatic Acinar Unit Formation and Centroacinar Cell Differentiation

doi: 10.1016/j.jcmgh.2014.12.004

Figure Lengend Snippet: Information about Primary Antibodies and Lectin Dye

Article Snippet: Fluorescein-Bauhenia , Purpurea lectin , Vector Labs, Burlingame, CA , FL-1281 , Q0910 , 1:100.

Techniques: Plasmid Preparation

Parabiotic demonstration of blood-borne CD34+ endothelial cells on Day 7 after pneumonectomy. (A) Schematic of experimental design involving a left pneumonectomy in the wild-type parabiont of wild-type/GFP+ parabiotic twins (C57/B6). Parabiosis was established for 28 days prior to left pneumonectomy. The remaining lung was studied 7 days after pneumonectomy. (B) Anti-GFP avidin–biotin complex (ABC) immunohistochemical staining demonstrated GFP+ cells within the alveolar septae. Flow cytometry of the lung digests demonstrated GFP+ cells that were CD45− (C) and CD31+ (D). (E and F) Analysis of CD31+ lung endothelial cells demonstrated that most of the GFP expression occurred in the CD34+ endothelial cell population (P < 0.01; n = 5).

Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: CD34 + Progenitor to Endothelial Cell Transition in Post-Pneumonectomy Angiogenesis

doi: 10.1165/rcmb.2011-0249OC

Figure Lengend Snippet: Parabiotic demonstration of blood-borne CD34+ endothelial cells on Day 7 after pneumonectomy. (A) Schematic of experimental design involving a left pneumonectomy in the wild-type parabiont of wild-type/GFP+ parabiotic twins (C57/B6). Parabiosis was established for 28 days prior to left pneumonectomy. The remaining lung was studied 7 days after pneumonectomy. (B) Anti-GFP avidin–biotin complex (ABC) immunohistochemical staining demonstrated GFP+ cells within the alveolar septae. Flow cytometry of the lung digests demonstrated GFP+ cells that were CD45− (C) and CD31+ (D). (E and F) Analysis of CD31+ lung endothelial cells demonstrated that most of the GFP expression occurred in the CD34+ endothelial cell population (P < 0.01; n = 5).

Article Snippet: To identify vessel integration, three endothelial-binding lectins were injected into the circulation: biotinylated Lycopersicon esculentum lectin (LEL), biotinylated Bandeiraea simplicifolia -1 lectin (BS-1), and biotinylated Ricinus communis agglutinin (RCA), purchased from Vector Laboratories (Burlingame, CA).

Techniques: Avidin-Biotin Assay, Immunohistochemical staining, Staining, Flow Cytometry, Expressing

Intravascular labeling of vascular lining cells of mice after pneumonectomy. Biotinylated lectins were injected into tail vein of the pneumonectomized mice on Day 7, and allowed to circulate for 10 minutes before flushing and harvest. Digested lung cells were stained with anti-CD45 mAb, anti-CD31 mAb, anti-CD34 mAb, and avidin-PE-Cy7. Data were analyzed by flow cytometry (see Materials and Methods). (A–C) The CD31+ CD45− endothelial-cell population was studied with anti-CD34 (purple) and avidin-PE-Cy7 to detect lectin binding. (D–F) Dual-parameter histograms of anti-CD31 and the lectins Lycopersicon esculentum (LEL) (D), Bandeiraea simplicifolia-1 (BS-1) (E), and Ricinus communis agglutinin (RCA) (F). (G–I) Single-parameter histograms of LEL, BS-1, and RCA binding are shown to facilitate comparisons of lectin binding with endothelial cells; gray indicates lectin control sample (C). CD34+ endothelial cells (purple) and CD34− endothelial cells (blue) are compared with lectin controls (gray).

Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: CD34 + Progenitor to Endothelial Cell Transition in Post-Pneumonectomy Angiogenesis

doi: 10.1165/rcmb.2011-0249OC

Figure Lengend Snippet: Intravascular labeling of vascular lining cells of mice after pneumonectomy. Biotinylated lectins were injected into tail vein of the pneumonectomized mice on Day 7, and allowed to circulate for 10 minutes before flushing and harvest. Digested lung cells were stained with anti-CD45 mAb, anti-CD31 mAb, anti-CD34 mAb, and avidin-PE-Cy7. Data were analyzed by flow cytometry (see Materials and Methods). (A–C) The CD31+ CD45− endothelial-cell population was studied with anti-CD34 (purple) and avidin-PE-Cy7 to detect lectin binding. (D–F) Dual-parameter histograms of anti-CD31 and the lectins Lycopersicon esculentum (LEL) (D), Bandeiraea simplicifolia-1 (BS-1) (E), and Ricinus communis agglutinin (RCA) (F). (G–I) Single-parameter histograms of LEL, BS-1, and RCA binding are shown to facilitate comparisons of lectin binding with endothelial cells; gray indicates lectin control sample (C). CD34+ endothelial cells (purple) and CD34− endothelial cells (blue) are compared with lectin controls (gray).

Article Snippet: To identify vessel integration, three endothelial-binding lectins were injected into the circulation: biotinylated Lycopersicon esculentum lectin (LEL), biotinylated Bandeiraea simplicifolia -1 lectin (BS-1), and biotinylated Ricinus communis agglutinin (RCA), purchased from Vector Laboratories (Burlingame, CA).

Techniques: Labeling, Injection, Staining, Avidin-Biotin Assay, Flow Cytometry, Binding Assay

Collagen I, collagen III, and TGF-β expression in the cardiac tissues of three groups of rats fed different diets. (A) Representative immunohistochemical staining for collagen I, collagen III, and TGF-β. Arrows indicate positively stained cells. (B) Bar graph showing collagen I, collagen III, and TGF-β positive cells. Data are presented as the mean ± SEM; n = 3 in each group. * P < 0.05 vs HFD group. (C) Immunoblotting for collagen I, collagen III, and TGF-β protein expression in cardiac tissues. (D) The bar graph shows the quantification of collagen I, collagen III, and TGF-β protein expression. Data are presented as the mean ± SEM; n = 3 in each group. * P < 0.05 vs HFD group.

Journal: Heliyon

Article Title: Luteolin reduces cardiac damage caused by hyperlipidemia in Sprague-Dawley rats

doi: 10.1016/j.heliyon.2023.e17613

Figure Lengend Snippet: Collagen I, collagen III, and TGF-β expression in the cardiac tissues of three groups of rats fed different diets. (A) Representative immunohistochemical staining for collagen I, collagen III, and TGF-β. Arrows indicate positively stained cells. (B) Bar graph showing collagen I, collagen III, and TGF-β positive cells. Data are presented as the mean ± SEM; n = 3 in each group. * P < 0.05 vs HFD group. (C) Immunoblotting for collagen I, collagen III, and TGF-β protein expression in cardiac tissues. (D) The bar graph shows the quantification of collagen I, collagen III, and TGF-β protein expression. Data are presented as the mean ± SEM; n = 3 in each group. * P < 0.05 vs HFD group.

Article Snippet: The PVDF membranes were blocked with 5% milk and incubated with the following primary antibodies at 4 °C overnight: primary antibodies against TGF-β (rabbit anti -TGF-β antibody, 1:1000; Proteintech, Wuhan, China), collagen I (rabbit anti-collagen I antibody, 1:1000; Proteintech), collagen III (rabbit anti-collagen III antibody, 1:1000; Proteintech), LOX-1 (rabbit anti-LOX-1 antibody, 1:500; Abcam), CD36 (rabbit anti-CD36 antibody, 1:1000; Proteintech), and β-actin ( anti -β-actin 1:1000; Cell Signaling Technology).

Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot

CD36 and LOX-1 expression in the cardiac tissues of three groups of rats fed different diets. (A) Representative immunohistochemical staining for CD36 and LOX-1. Arrows indicate positively stained cells. (B) Bar graph showing CD36 and LOX-1 positive cells. Data are presented as the mean ± SEM; n = 3 in each group. * P < 0.05 vs HFD group. (C) Immunoblotting for CD36 and LOX-1 protein expression in cardiac tissues. (D) The bar graph shows the quantification of CD36 and LOX-1 protein expression. Data are presented as the mean ± SEM; n = 3 in each group. * P < 0.05 vs HFD group, ** P < 0.01 vs HFD group.

Journal: Heliyon

Article Title: Luteolin reduces cardiac damage caused by hyperlipidemia in Sprague-Dawley rats

doi: 10.1016/j.heliyon.2023.e17613

Figure Lengend Snippet: CD36 and LOX-1 expression in the cardiac tissues of three groups of rats fed different diets. (A) Representative immunohistochemical staining for CD36 and LOX-1. Arrows indicate positively stained cells. (B) Bar graph showing CD36 and LOX-1 positive cells. Data are presented as the mean ± SEM; n = 3 in each group. * P < 0.05 vs HFD group. (C) Immunoblotting for CD36 and LOX-1 protein expression in cardiac tissues. (D) The bar graph shows the quantification of CD36 and LOX-1 protein expression. Data are presented as the mean ± SEM; n = 3 in each group. * P < 0.05 vs HFD group, ** P < 0.01 vs HFD group.

Article Snippet: The PVDF membranes were blocked with 5% milk and incubated with the following primary antibodies at 4 °C overnight: primary antibodies against TGF-β (rabbit anti -TGF-β antibody, 1:1000; Proteintech, Wuhan, China), collagen I (rabbit anti-collagen I antibody, 1:1000; Proteintech), collagen III (rabbit anti-collagen III antibody, 1:1000; Proteintech), LOX-1 (rabbit anti-LOX-1 antibody, 1:500; Abcam), CD36 (rabbit anti-CD36 antibody, 1:1000; Proteintech), and β-actin ( anti -β-actin 1:1000; Cell Signaling Technology).

Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot